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Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each <t>microarray</t> chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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arabidopsis thaliana ath1 microarray  (Thermo Fisher)
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Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each <t>microarray</t> chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each <t>microarray</t> chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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ath1 genechip microarrays  (Thermo Fisher)
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Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each <t>microarray</t> chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix Arabidopsis ATH1 chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.

Journal: International Journal of Molecular Sciences

Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

doi: 10.3390/ijms251910850

Figure Lengend Snippet: Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix Arabidopsis ATH1 chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.

Article Snippet: These data were collected from nearly 1000 chips representing 70 Arabidopsis Affymetrix ATH1 microarray experiments.

Techniques: Expressing, Starch, Activity Assay, Microarray

Expression correlations among genes within the starch metabolic network. Expression correlations among starch metabolic genes were evaluated by 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG . Pearson correlation values are color-coded. Vertical bars (labeled #1, group 1; #2, group 2; and #3, group 3) denote the three groups of genes with the highest correlations. Group 1 represents genes involved in starch degradation, Group 2 includes genes primarily associated with starch synthesis, and Group 3 comprises genes involved in both starch synthesis and carbohydrate metabolism. Details for each group are described in the text.

Journal: International Journal of Molecular Sciences

Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

doi: 10.3390/ijms251910850

Figure Lengend Snippet: Expression correlations among genes within the starch metabolic network. Expression correlations among starch metabolic genes were evaluated by 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG . Pearson correlation values are color-coded. Vertical bars (labeled #1, group 1; #2, group 2; and #3, group 3) denote the three groups of genes with the highest correlations. Group 1 represents genes involved in starch degradation, Group 2 includes genes primarily associated with starch synthesis, and Group 3 comprises genes involved in both starch synthesis and carbohydrate metabolism. Details for each group are described in the text.

Article Snippet: These data were collected from nearly 1000 chips representing 70 Arabidopsis Affymetrix ATH1 microarray experiments.

Techniques: Expressing, Starch, Microarray, Labeling

Gene expression correlated with the expression of the DPE2 gene. Gene-expression profiles of nine genes ( PTPKIS1 / SEX4 , GWD1 , ISA3 , GWD3 , ISA2 , PHS2 , PHS1 , AT1G06460, and AT2G31040) correlated to the expression profile of DPE2 (Pearson’s correlation coefficients of between 0.72 and 0.84). Gene expression was evaluated at the level of RNA accumulation from 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG ; the average expression level for each chip is 6.6.

Journal: International Journal of Molecular Sciences

Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

doi: 10.3390/ijms251910850

Figure Lengend Snippet: Gene expression correlated with the expression of the DPE2 gene. Gene-expression profiles of nine genes ( PTPKIS1 / SEX4 , GWD1 , ISA3 , GWD3 , ISA2 , PHS2 , PHS1 , AT1G06460, and AT2G31040) correlated to the expression profile of DPE2 (Pearson’s correlation coefficients of between 0.72 and 0.84). Gene expression was evaluated at the level of RNA accumulation from 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG ; the average expression level for each chip is 6.6.

Article Snippet: These data were collected from nearly 1000 chips representing 70 Arabidopsis Affymetrix ATH1 microarray experiments.

Techniques: Expressing, Microarray